F
-AI
Fluorescence Image Analyzer
browser edition
No installation · No data upload
All processing in your browser
Image input
Click to upload or drag & drop
JPG · PNG · up to any size
Signal channel
Channel
green
0 = red · 1 = green (CellMask) · 2 = blue (DAPI)
Segmentation
Nucleus threshold
auto
0 = Otsu auto-detect (recommended)
Min nucleus area (px)
150
Region geometry
Ring width (px)
8
Membrane proxy thickness
Cell dilation (px)
18
Cell proxy expansion from nucleus
Analyze image
Download results CSV
Original
Segmentation overlay
M/C heatmap
▴
Upload a fluorescence image to begin
CellMask, DAPI, or any single-channel IF image
Image summary
Cells detected
—
Median M/C ratio
—
ring / cytoplasm
Median N/C ratio
—
nucleus / cytoplasm
Per-cell results
#
Area (px)
Ring mean
Cyto mean
M/C ratio
N/C ratio
Localization
Confidence